Toxicity of Imidacloprid on Peripheral Blood Lymphocytes by MTT Assay and the Ameliorative Effect of Extract of Tinospora cordifolia (Gilloe) Extract

Imidacloprid (IMI) is a widely used insecticide which has specific affinity for insect neonicotinoid acetylcholine receptors. Like all insecticides are in excess it tends to bioaccumulate the environment. So was thought worthwhile study its cytotoxicity human peripheral blood lymphocytes concentrations ranging from 1.5mM 4mM after 2 hours and 18 exposure by MTT method. Trypan blue test also determine percentage of living cells. The ameliorative effect an extract stem water ethanolic leaves Tinospora cordifolia (Thunb.) Miers, studied. viability showed fall with increasing at hours. After IMI only, significant dose dependent drop. conducted. Addition raised significantly incubation. In fact this increase greatest 3.5mM concentration drug. maximum leaf cell incubation as compared values obtained only Thus considerable loss seen drug selected but herbal extracts seem help make damage less marked. cells rise when incubated confirming supportive action proliferation. However, taking into account evident caused considerably dilute tested, caution needed prevent over pesticide while spraying.